Development of a System for Research in the Field of Laser Cytometry

Purpose of research. In this paper, we propose a block diagram of a laser cytometry system designed for non-invasive hematological tests. The relevance of this work lies in the growing interest in one's own health, as well as the need to monitor changes in the patient's condition. The aim of this work is to develop a portable laser cytometry system, which is a diagnostic complex and based on an optoacoustic effect - the conversion of optical radiation into thermo-acoustic waves in a medium at a laser wavelength A = 1064 nm. This effect will allow you to obtain information about the state, shape and size of uniform blood cells, to determine the degree of aggregation of erythrocytes and the level of saturation. Flow cytometry is a technology for the rapid measurement of cell parameters and processes occurring in it. The flow cytometry technique consists in identifying the processes of absorption and scattering of light from a laser beam when cells pass through it in a fluid flow.

Methods. The main tasks of this study are to substantiate the structural diagram of the laser cytometry system, determine the constituent parts of the device being developed and identify the connections between them. In the developed block diagram, two measurement channels can be distinguished: optoacoustic and additional, based on Doppler. The signal coming from the additional measuring channel provides information about the blood velocity, which will make it possible to make corrections to the signal received from the optoacoustic channel.

Results. In the proposed system, it is necessary to take into account the corrections for the blood velocity. In the course of this work, a structural diagram of a laser cytometry system was proposed for non-invasive blood analysis. The block diagram of the system was implemented in the computer-aided design system KOMPAS-3D. An analysis carried out using a laser cytometry system can provide information about various diseases and pathological changes in the composition of the blood.

Conclusion. The developed laser cytometry system is distinguished from other hemoanalyzers by its non- invasiveness, which can serve as an excellent advantage in conditions where invasive intervention is impossible or is fraught with certain difficulties

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